Vinante F, Morosato L, Siviero F, Nadali G, Rigo A, Veneri D, de Sabata D, Vincenzi C, Chilosi M, Semenzato G
Department of Hematology, Verona University School of Medicine, Italy.
Haematologica. 1994 Sep-Oct;79(5):413-9.
Epstein-Barr virus (EBV) infection in infectious mononucleosis (IM) is associated with lymphocyte activation leading to the expansion of cells expressing activation-associated antigens. Most of these antigens are released as soluble molecules in vitro and in vivo.
We investigated the serum levels of the soluble forms of the CD8 (sCD8), p55-IL-2R alpha (sIL-2R alpha), and CD30 (sCD30) molecules in 55 patients following primary EBV infection. These data were compared with the phenotypic pattern of circulating lymphoid subsets.
In all cases at presentation, lymphocytosis, mainly characterized by the expansion of a CD8+, HLA-DR+, p75-IL-2R beta+, p55-IL-2R alpha- population, was associated with high levels of the investigated soluble molecules. Their mean values (+/- SD) were: 17,172 +/- 12,885 U/mL for sCD8 (vs 334 +/- 95 in controls), 2,922 +/- 2,813 U/mL for sIL-2R alpha (vs 331 +/- 115 in controls), and 477 +/- 451 U/mL for sCD30 (vs 4.9 +/- 6.4 in controls). Follow-up study (15 cases, up to 60 days) showed a progressive decline of all soluble molecules, associated with a reduction of activated CD8+/HLA-DR+/p75-IL-2R beta+ T-cells. By the 30th day, values of sIL-2R alpha and sCD30 (729 +/- 333 U/mL and 20 +/- 21 U/mL, respectively) were only slightly higher than in normal controls, whereas sCD8 levels remained consistently higher (1,777 +/- 1,385 U/mL, p < .001).
sCD8, sIL-2Ra and sCD30 serum levels in IM reflect the total bulk and/or the activation-related events of infected and reactive cells. The variations in these soluble molecules during the follow-up provide useful information on the in vivo biological modifications occurring after EBV infection.
传染性单核细胞增多症（IM）中的爱泼斯坦-巴尔病毒（EBV）感染与淋巴细胞活化相关，导致表达活化相关抗原的细胞扩增。这些抗原中的大多数在体外和体内以可溶性分子的形式释放。
我们调查了55例原发性EBV感染患者血清中可溶性CD8（sCD8）、p55-IL-2Rα（sIL-2Rα）和CD30（sCD30）分子的水平。将这些数据与循环淋巴细胞亚群的表型模式进行比较。
在所有病例就诊时，淋巴细胞增多，主要表现为CD8 +、HLA-DR +、p75-IL-2Rβ +、p55-IL-2Rα - 细胞群的扩增，与所研究的可溶性分子的高水平相关。它们的平均值（±标准差）分别为：sCD8为17,172±12,885 U/mL（对照组为334±95），sIL-2Rα为2,922±2,813 U/mL（对照组为331±115），sCD30为477±451 U/mL（对照组为4.9±6.4）。随访研究（15例，长达60天）显示所有可溶性分子逐渐下降，与活化的CD8 + / HLA-DR + / p75-IL-2Rβ + T细胞减少相关。到第30天时，sIL-2Rα和sCD30的值（分别为729±333 U/mL和20±21 U/mL）仅略高于正常对照组，而sCD8水平仍持续较高（1,777±1,385 U/mL，p <.001）。
IM中sCD8、sIL-2Ra和sCD30血清水平反映了感染和反应性细胞的总量和/或与活化相关的事件。随访期间这些可溶性分子的变化为EBV感染后体内发生的生物学改变提供了有用信息。
