D'Cruz D, Direskeneli H, Khamashta M, Hughes G R
Bone and Joint Research Unit, St. Bartholomew's and the Royal London School of Medicine and Dentistry, UK.
J Rheumatol. 1999 Jan;26(1):103-9.
To examine 3 soluble circulating T cell antigens: interleukin 2 receptors (sIL-2r), CD4 (sCD4), and CD8 (sCD8) with von Willebrand factor antigen levels (vWF:Ag) and antiendothelial cell antibodies (AECA) as indices of endothelial involvement in patients with Wegener's granulomatosis (WG).
We studied 23 patients with WG, of whom 11 had active disease with renal involvement, and 20 healthy controls. sIL-2r, sCD4, sCD8, vWF:Ag, and AECA were measured by ELISA.
Median sIL-2r levels in patients were higher than controls (789.5 vs. 551 U/ml, p<0.01). sCD4 levels were higher in patients: 17.0 vs. 15.2 U/ml (p<0.005) and correlated with sIL-2r levels. sIL-2r and sCD4 levels correlated with disease activity scores, antineutrophil cytoplasmic antibodies (ANCA), C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), and neutrophil counts. sCD8 levels were also higher: 260.5 vs. 127 U/ml (p<0.001) and correlated with CRP levels but not with disease activity. vWF:Ag levels were elevated in patients (median 296% vs. controls 109%; p<0.001) and correlated with disease activity, ESR, CRP, ANCA titers, and serum creatinine levels. AECA levels did not differ from controls (5% vs. 7%).
Circulating levels of sIL-2r, sCD4, sCD8, and vWF:Ag are elevated in active WG, indicating T cell and endothelial activation. sIL-2r, sCD4, and vWF:Ag are potentially useful disease activity markers.
以血管性血友病因子抗原水平（vWF:Ag）和抗内皮细胞抗体（AECA）作为韦格纳肉芽肿（WG）患者内皮受累指标，检测3种可溶性循环T细胞抗原：白细胞介素2受体（sIL-2r）、CD4（sCD4）和CD8（sCD8）。
我们研究了23例WG患者，其中11例有肾脏受累的活动期疾病，以及20名健康对照者。采用酶联免疫吸附测定法（ELISA）检测sIL-2r、sCD4、sCD8、vWF:Ag和AECA。
患者的sIL-2r水平中位数高于对照者（789.5对551 U/ml，p<0.01）。患者的sCD4水平更高：17.0对15.2 U/ml（p<0.005），且与sIL-2r水平相关。sIL-2r和sCD4水平与疾病活动评分、抗中性粒细胞胞浆抗体（ANCA）、C反应蛋白（CRP）、红细胞沉降率（ESR）和中性粒细胞计数相关。sCD8水平也更高：260.5对127 U/ml（p<0.001），且与CRP水平相关，但与疾病活动无关。患者的vWF:Ag水平升高（中位数296%对对照者109%；p<0.001），且与疾病活动、ESR、CRP、ANCA滴度和血清肌酐水平相关。AECA水平与对照者无差异（5%对7%）。
活动期WG患者循环中的sIL-2r、sCD4、sCD8和vWF:Ag水平升高，表明T细胞和内皮细胞活化。sIL-2r、sCD4和vWF:Ag可能是有用的疾病活动标志物。
