Frydecka I, Rusiecka M, Kuliczkowski K, Kornafel J
Department of Hematology, Medical Academy, Wroclaw, Poland.
Arch Immunol Ther Exp (Warsz). 1996;44(2-3):123-6.
The phenotype peripheral blood lymphocytes, soluble interleukin 2 receptor alpha (sIL-2R alpha) and soluble CD8 (sCD8) were studied in 30 patients with gynecological malignancies before and after ratiotherapy. The absolute count of CD3+, CD4+, CD8+, CD19+ and CD25+ lymphocytes before treatment was within normal range. The levels of serum sIL-2R alpha and sCD8 were significantly increased before treatment compared to normal controls. After treatment the absolute number of CD3+, CD4+ and CD19+ lymphocyte subsets significantly decreased. The levels of sIL-2 alpha and sCD8 corrected according to absolute lymphocyte count reached normal range. The decrease of tumor mass correlated with the serum levels of sIL-2R alpha and sCD8. Further studies are needed to evaluate the usefulness of these tests in the prognosis and treatment of these diseases. Our study suggests that sIL-2R alpha and sCD8 in our patients probably has not been originating from the proteolytic cleavage of membrane bound receptors from peripheral blood mononuclear cells (PBMC). It can be speculated that sIL-2R alpha and sCD8 originated in these patients from activated tumor-infiltrating lymphocytes or may arise from separate alternatively spliced mRNAs coding for soluble vs. membrane forms.
对30例妇科恶性肿瘤患者放疗前后的外周血淋巴细胞表型、可溶性白细胞介素2受体α（sIL-2Rα）和可溶性CD8（sCD8）进行了研究。治疗前CD3 +、CD4 +、CD8 +、CD19 +和CD25 +淋巴细胞的绝对计数在正常范围内。与正常对照组相比，治疗前血清sIL-2Rα和sCD8水平显著升高。治疗后，CD3 +、CD4 +和CD19 +淋巴细胞亚群的绝对数量显著下降。根据淋巴细胞绝对计数校正后的sIL-2α和sCD8水平达到正常范围。肿瘤肿块的缩小与血清sIL-2Rα和sCD8水平相关。需要进一步研究来评估这些检测在这些疾病的预后和治疗中的作用。我们的研究表明，我们患者中的sIL-2Rα和sCD8可能并非源自外周血单核细胞（PBMC）膜结合受体的蛋白水解裂解。可以推测，这些患者中的sIL-2Rα和sCD8源自活化的肿瘤浸润淋巴细胞，或者可能来自编码可溶性与膜结合形式的不同剪接mRNA。
