堪萨斯分枝杆菌感染的人单核细胞中肿瘤坏死因子α和白细胞介素6的释放：与鸟分枝杆菌的比较。

Release of TNF alpha and IL6 from human monocytes infected with Mycobacterium kansasii: a comparison to Mycobacterium avium.

作者信息

Stauffer F, Petrow E P, Burgmann H, Graninger W, Georgopoulos A

机构信息

Dept. of Chemotherapy and Infectious Diseases, University of Vienna, Austria.

出版信息

Infection. 1994 Sep-Oct;22(5):326-9. doi: 10.1007/BF01715538.

DOI:10.1007/BF01715538

PMID:7843810

Abstract

Tumor necrosis factor alpha (TNF alpha) and interleukin 6 (IL6) are cytokines with a varied spectrum of inflammatory and immunological activities, for example modulation of acute phase proteins, fever and stimulation of B-lymphocytes. Mycobacterium avium has been shown to stimulate the release of TNF alpha and IL6 from cultured human monocytes and macrophages into the cell supernatant. Cultured human monocytes were infected with Mycobacterium kansasii and M. avium. The concentrations of TNF alpha and IL6 were measured in the supernatant. Monocytes infected with M. kansasii produced significantly lower amounts of TNF alpha (34.8 +/- 20.3 pg/ml) and IL6 (12.0 +/- 8.9 pg/ml) compared to monocytes infected with M. avium (198.3 +/- 171.7 pg/ml and 63.2 +/- 37.6 pg/ml respectively). The extent of cytokine production might be relevant for the clinical manifestation of mycobacterial disease.

摘要

肿瘤坏死因子α（TNFα）和白细胞介素6（IL6）是具有多种炎症和免疫活性的细胞因子，例如调节急性期蛋白、发热以及刺激B淋巴细胞。已证明鸟分枝杆菌可刺激培养的人单核细胞和巨噬细胞将TNFα和IL6释放到细胞上清液中。用堪萨斯分枝杆菌和鸟分枝杆菌感染培养的人单核细胞。测量上清液中TNFα和IL6的浓度。与感染鸟分枝杆菌的单核细胞（分别为198.3±171.7 pg/ml和63.2±37.6 pg/ml）相比，感染堪萨斯分枝杆菌的单核细胞产生的TNFα（34.8±20.3 pg/ml）和IL6（12.0±8.9 pg/ml）量显著更低。细胞因子产生的程度可能与分枝杆菌病的临床表现相关。