Labonté P, Mounir S, Talbot P J
Virology Research Center, Institut Armand-Frappier, Université du Québec, Laval, Canada.
J Gen Virol. 1995 Feb;76 ( Pt 2):431-5. doi: 10.1099/0022-1317-76-2-431.
The complete nucleotide sequence of the ns2 gene of human coronavirus OC43 (HCV-OC43) was determined. Sequence analysis revealed an open reading frame that could encode a protein of 278 amino acids, with an estimated molecular mass of 32.2 kDa. Six potential phosphorylation sites are present but no sites of N-glycosylation were found. The amino acid sequence of the HCV-OC43 ns2 protein shows 92% identity with that of the Mebus strain of bovine coronavirus (BCV). However, a stretch of nine consecutive amino acids near the C terminus is completely different, causing it to be very hydrophilic, which contrasts with the hydrophobic nature of this region in BCV. As shown by immunofluorescence with a monospecific antiserum, the ns2 protein was expressed in the cytoplasm of HCV-OC43-infected HRT-18 cells.
测定了人冠状病毒OC43（HCV-OC43）ns2基因的完整核苷酸序列。序列分析显示有一个开放阅读框，可编码一个由278个氨基酸组成的蛋白质，估计分子量为32.2 kDa。存在六个潜在的磷酸化位点，但未发现N-糖基化位点。HCV-OC43 ns2蛋白的氨基酸序列与牛冠状病毒（BCV）的Mebus株的氨基酸序列有92%的同一性。然而，靠近C末端的一段连续九个氨基酸完全不同，使其具有很强的亲水性，这与BCV中该区域的疏水性形成对比。用单特异性抗血清进行免疫荧光检测表明，ns2蛋白在HCV-OC43感染的HRT-18细胞的细胞质中表达。
