Garner M, Reglinski J, Smith W E, Stewart M J
Department of Pure and Applied Chemistry, Strathclyde University, Glasgow, Scotland, U.K.
J Inorg Biochem. 1994 Dec;56(4):283-90. doi: 10.1016/0162-0134(94)85108-5.
The interactions of citrate reduced colloids (Ag, Au, and Bi) with intact erythrocytes and erythrocyte lysate have been studied by 1H spin echo NMR. Silver colloid is observed to induce cellular depletion of cytosolic glutathione and bismuth colloid induces cytosolic glutathione oxidation in the intact cell. In comparison, there is no detectable effect with gold colloid. With red cell lysate the three colloids all remove glutathione from the spectrum. The metal salts AgNO3 and NaAuCl4 both oxidize intracellular glutathione to diglutathione whereas BiO(NO3) has no effect. Thus colloidal preparations have a different reactivity to their parent metal salts. The differences observed between the three types of colloids (silver, gold, and bismuth) are unique to the colloids studied. None of the colloids studied were biologically inert in the erythrocyte model used.
通过1H自旋回波核磁共振研究了柠檬酸盐还原胶体（银、金和铋）与完整红细胞及红细胞裂解物之间的相互作用。观察到银胶体可诱导完整细胞中胞质谷胱甘肽的细胞内耗竭，铋胶体可诱导完整细胞中胞质谷胱甘肽氧化。相比之下，金胶体没有可检测到的影响。对于红细胞裂解物，这三种胶体都会使谷胱甘肽从光谱中消失。金属盐硝酸银和氯金酸钠都会将细胞内谷胱甘肽氧化为双谷胱甘肽，而硝酸铋则没有影响。因此，胶体制剂与其母体金属盐具有不同的反应性。在研究的三种胶体（银、金和铋）之间观察到的差异是所研究胶体特有的。在所使用的红细胞模型中，所研究的胶体均无生物惰性。
