Department of biology, Faculty of sciences and Arts Khulais, University of Jeddah, Jeddah, Saudi Arabia.
Department of Microbial Biotechnology, Genetic Engineering & Research Institute, Sadat University, Sadat city, Egypt.
Sci Rep. 2019 Sep 10;9(1):13071. doi: 10.1038/s41598-019-49444-y.
Using aqueous cyanobacterial extracts in the synthesis of silver nanoparticle is looked as green, ecofriendly, low priced biotechnology that gives advancement over both chemical and physical methods. In the current study, an aqueous extract of Oscillatoria limnetica fresh biomass was used for the green synthesis of Ag-NPs, since O. limnetica extract plays a dual part in both reducing and stabilizing Oscillatoria-silver nanoparticles (O-AgNPs). The UV-Visible absorption spectrum, Fourier transforms infrared (FT-IR), transmission electron microscopy (TEM) and scanning electron microscope (SEM) were achieved for confirming and characterizing the biosynthesized O-AgNPs. TEM images detected the quasi-spherical Ag-NPs shape with diverse size ranged within 3.30-17.97 nm. FT-IR analysis demonstrated the presence of free amino groups in addition to sulfur containing amino acid derivatives acting as stabilizing agents as well as the presence of either sulfur or phosphorus functional groups which possibly attaches silver. In this study, synthesized Ag-NPs exhibited strong antibacterial activity against multidrug-resistant bacteria (Escherichia coli and Bacillus cereus) as well as cytotoxic effects against both human breast (MCF-7) cell line giving IC50 (6.147 µg/ml) and human colon cancer (HCT-116) cell line giving IC50 (5.369 µg/ml). Hemolytic activity of Ag-NPs was investigated and confirmed as being non- toxic to human RBCs in low concentrations.
利用水生生蓝细菌提取物合成银纳米颗粒被视为绿色、环保、低成本的生物技术，优于化学和物理方法。在本研究中，使用新鲜的颤藻（Oscillatoria limnetica）生物量的水提取液来绿色合成 Ag-NPs，因为颤藻提取物在还原和稳定颤藻-银纳米颗粒（O-AgNPs）方面发挥双重作用。通过紫外可见吸收光谱、傅里叶变换红外光谱（FT-IR）、透射电子显微镜（TEM）和扫描电子显微镜（SEM）来确认和表征生物合成的 O-AgNPs。TEM 图像检测到准球形 Ag-NPs 形状，尺寸范围在 3.30-17.97nm 之间。FT-IR 分析表明，除了含硫氨基酸衍生物作为稳定剂的游离氨基外，还存在硫或磷功能基团，可能与银结合。在这项研究中，合成的 Ag-NPs 对多药耐药菌（大肠杆菌和蜡状芽孢杆菌）表现出很强的抗菌活性，对人乳腺癌（MCF-7）细胞系和人结肠癌细胞（HCT-116）系均表现出细胞毒性作用，IC50 分别为 6.147µg/ml 和 5.369µg/ml。Ag-NPs 的溶血活性也进行了研究和证实，在低浓度下对人 RBC 无毒性。
