Iyama T, Takasuga A, Azuma M
Department of Health Science, Osaka Kyoiku University, Japan.
Int J Vitam Nutr Res. 1996;66(4):301-5.
Mice were supplemented with beta-carotene in beverage for 10 days. After the supplement, beta-carotene accumulated mainly in the liver and to some extent in the blood plasma, kidney and lung. The beta-carotene administration was associated with an increase in the amount of retinyl ester and retinol in the liver, but not in the amount of retinol in blood plasma. Lipid peroxidation in vivo was induced by the injection of carbon tetrachloride under the dorsal skin of mice. As an index of lipid peroxidation, thiobarbituric acid-reacting substances (TBARS) were assayed in urine and tissue homogenates. Urine and kidney TBARS were reduced by the supplementation of beta-carotene. The amounts of TBARS in kidney, liver and lung, decreased with increasing amounts of beta-carotene accumulated in these tissues, i.e. inverse correlations were obtained. These results indicate that beta-carotene can suppress lipid peroxidation in mouse tissue.
给小鼠在饮料中补充β-胡萝卜素10天。补充后，β-胡萝卜素主要在肝脏中蓄积，在血浆、肾脏和肺中也有一定程度的蓄积。给予β-胡萝卜素与肝脏中视黄酯和视黄醇含量的增加有关，但与血浆中视黄醇含量无关。通过在小鼠背部皮肤下注射四氯化碳诱导体内脂质过氧化。作为脂质过氧化的指标，在尿液和组织匀浆中检测硫代巴比妥酸反应物质（TBARS）。补充β-胡萝卜素可降低尿液和肾脏中的TBARS。肾脏、肝脏和肺中的TBARS量随着这些组织中β-胡萝卜素蓄积量的增加而减少，即呈负相关。这些结果表明，β-胡萝卜素可抑制小鼠组织中的脂质过氧化。
