Maebashi K, Itoyama T, Uchida K, Suegara N, Yamaguchi H
Research Center for Medical Mycology, Teikyo University, Tokyo, Japan.
J Med Vet Mycol. 1994;32(5):349-59. doi: 10.1080/02681219480000471.
In an attempt to develop an animal model of cutaneous candidiasis useful for the pre-clinical evaluation of antifungal drugs, experimental cutaneous Candida albicans infections were produced in mature and immature guinea-pigs treated with prednisolone. The morbidity of this model in terms of the extent and the duration of the superficial infection was compared with that of two other reported models: those produced by alloxan treatment, or the use of occlusive dressings. Infected animals were also included which received neither of these treatments. The Candida infection continued steadily for 14 days or more in both mature and immature animal groups treated subcutaneously with prednisolone and appeared to be more suitable than that produced with alloxan treatment or under occlusive dressings. In prednisolone-induced infection, most of the micro-organisms were confined to the epidermis and there was no evidence of their dermal penetration during the 14 day experimental period. In view of these facts, we attempted a more quantitative method by counting viable Candida in order to evaluate antimycotics in a shorter therapy period during which animals would receive less exposure to the vehicle. These findings appear to indicate that the experimental model of cutaneous C. albicans infection produced in prednisolone-treated mature and immature guinea-pigs would be useful for studies on the therapeutic efficacy of antifungal agents against cutaneous candidiasis.
为了建立一种有助于抗真菌药物临床前评估的皮肤念珠菌病动物模型，在用泼尼松龙治疗的成熟和未成熟豚鼠身上制造了实验性皮肤白色念珠菌感染。将该模型在浅表感染的范围和持续时间方面的发病率与其他两种已报道的模型进行了比较：用四氧嘧啶治疗产生的模型，或使用封闭敷料产生的模型。还纳入了未接受这两种治疗的感染动物。在用泼尼松龙皮下治疗的成熟和未成熟动物组中，念珠菌感染持续稳定14天或更长时间，似乎比用四氧嘧啶治疗或在封闭敷料下产生的感染更合适。在泼尼松龙诱导的感染中，大多数微生物局限于表皮，在14天的实验期内没有它们真皮渗透的证据。鉴于这些事实，我们尝试了一种更定量的方法，即通过计数存活的念珠菌来评估抗真菌药物，以便在较短的治疗期内进行评估，在此期间动物接触赋形剂的机会较少。这些发现似乎表明，在用泼尼松龙治疗的成熟和未成熟豚鼠身上产生的皮肤白色念珠菌感染实验模型，将有助于研究抗真菌剂对皮肤念珠菌病的治疗效果。
