Mushiake S, Nakayama M, Imura K, Harada T, Okada S, Tohyama M
Department of Pediatrics, Osaka University Medical School, Japan.
J Pediatr Surg. 1994 Nov;29(11):1479-82. doi: 10.1016/0022-3468(94)90149-x.
The use of two-dimensional demonstration of myenteric nerve plexus for the morphological estimation of pseudo-Hirschsprung's disease is introduced. By fluorescent immunohistochemistry for S-100 protein on flat-mounted frozen sections, a decrease in the amount of ganglionic plexuses in the small intestinal segments in cases of pseudo-Hirschsprung's disease was able to be distinguished from normoganglionic plexuses in control cases. Moreover, a morphological difference between the two types of cases was clearly demonstrated. This method may be an indispensable tool in the reliable diagnosis of pseudo-Hirschsprung's disease.
