Vorob'eva L I, Khodzhaev E Iu, Ponomareva G M, Ambrosov I V
Mikrobiologiia. 1994 Jul-Aug;63(4):630-6.
Cell free extract from Propionibacterium shermanii VKM-101 partially reactivates Escherichia coli AB 1157 irradiated by UV light. Fractionation of extract followed by the estimation of protective effect of fractions showed that this effect is linked to two fractions of soluble proteins. The fraction of cell walls, ribosomes and nucleic acids were poorly effective. Two active protein fractions (I-20-40% (NH4)SO4 and II-60-80% (NH4)2SO4) were separated by HPLC chromatography into 7 and 8 subfractions respectively. The activity was localised in subfraction N4 (fraction I) and N5.6 (fraction II).
费氏丙酸杆菌VKM-101的无细胞提取物可部分恢复经紫外线照射的大肠杆菌AB 1157的活性。对提取物进行分级分离并评估各组分的保护作用后发现，这种作用与两个可溶性蛋白质组分有关。细胞壁、核糖体和核酸组分的效果不佳。通过高效液相色谱法将两个活性蛋白质组分（I-20-40%硫酸铵和II-60-80%硫酸铵）分别分离成7个和8个子组分。活性定位于子组分N4（组分I）和N5.6（组分II）。
