Godin I, Dieterlen-Lièvre F, Cumano A
Institut d'Embryologie Cellulaire et Moléculaire du Centre National de la Recherche Scientifique et du Collège de France, Nogent-sur-Marne.
Proc Natl Acad Sci U S A. 1995 Jan 31;92(3):773-7. doi: 10.1073/pnas.92.3.773.
We show by an in vitro approach that multipotent hemopoietic cells can be detected in the body of the mouse embryo between the stages of 10-25 somites (8.5-9.5 days of gestation)--i.e., prior to liver colonization (28-32 pairs of somites). Interestingly, hemopoietic cells appear in parallel in this location, the paraaortic splanchnopleura, and in the yolk sac, where they represent a new generation by reference to the primitive hemopoietic stem cells. Lymphoid cell clones, which could differentiate into mature B cells, were obtained from yolk sac and paraaortic splanchnopleura cell preparations but not from other tissues of the embryonic body. These B-cell precursors were first detected around the stage of 10 somites; thereafter, their initial minute numbers increased in parallel in the yolk sac and the paraaortic splanchnopleura, suggesting that their emergence in the two sites was simultaneous. By single cell manipulation, we show that these precursors can generate B and T lymphocytes and myeloid cells; these precursors can thus be defined as multipotent hemopoietic cells.
我们通过体外实验方法表明，在小鼠胚胎10至25体节阶段（妊娠8.5至9.5天）——即在肝脏定植之前（28至32对体节），可在小鼠胚胎体内检测到多能造血细胞。有趣的是，造血细胞在这个位置——主动脉旁脏壁中胚层，以及卵黄囊中同时出现，相对于原始造血干细胞而言，它们在那里代表新一代细胞。能够分化为成熟B细胞的淋巴细胞克隆是从卵黄囊和主动脉旁脏壁细胞制剂中获得的，而非从胚胎体的其他组织中获得。这些B细胞前体最早在10体节阶段左右被检测到；此后，它们最初的微量数量在卵黄囊和主动脉旁脏壁中平行增加，这表明它们在这两个部位的出现是同时的。通过单细胞操作，我们表明这些前体可以产生B细胞、T淋巴细胞和髓系细胞；因此，这些前体可被定义为多能造血细胞。
