Zito F A, Gadaleta C D, Salvatore C, Filotico R, Labriola A, Marzullo A, Prete F, Marzullo F
Division of Anatomical Pathology and Cytology, National Cancer Research Institute of Bari, Italy.
Acta Cytol. 1995 Jan-Feb;39(1):93-9.
A modification of the cell block technique, useful in processing material obtained by fine needle aspiration (FNA), is described. Four hundred six aspirates, obtained from 333 consecutive patients, were studied after immediate fixation in 4% buffered paraformaldehyde. Conventional histochemical and immunohistochemical staining methods were used. Histologic verification of the cytologic diagnoses made by FNA was possible in 67 cases. The overall accuracy was 97%, with a sensitivity of 95% and specificity of 100%. A major disadvantage of the cell block technique is time. Therefore, even if this technique increases the accuracy of cytologic diagnosis, its routine use is impractical because the delay in diagnosis when compared with smears may be considerable. The cell block technique is a valuable method, particularly when immunohistochemical staining for a battery of markers is required.
本文描述了一种细胞块技术的改良方法，该方法在处理细针穿刺（FNA）获取的材料时很有用。对连续333例患者的406份穿刺样本在立即固定于4%缓冲多聚甲醛后进行了研究。采用了传统的组织化学和免疫组织化学染色方法。67例病例中通过FNA做出的细胞学诊断能够得到组织学验证。总体准确率为97%，敏感性为95%，特异性为100%。细胞块技术的一个主要缺点是耗时。因此，即使该技术提高了细胞学诊断的准确性，但与涂片相比诊断延迟可能相当大，其常规使用并不实际。细胞块技术是一种有价值的方法，特别是在需要对一系列标志物进行免疫组织化学染色时。
