Atkinson P W, King J A, Hynes M J
Department of Genetics, University of Melbourne, Parkville, Victoria, Australia.
Curr Genet. 1985;10(2):133-8. doi: 10.1007/BF00636478.
The amdA gene is one of a number of transacting regulatory genes controlling expression of the amdS gene in A. nidulans. A polypeptide of approximately 42,000 molecular weight has been found to be synthesized constitutively in amdA mutant strains and to be acetate inducible. A lambda clone containing a gene, called aciA, coding for this polypeptide has been isolated using differential screening with cDNA probes. Two acetate inducible RNA species have been identified by probing Northern blots with aciA containing probes. It is suggested that the amdA gene is a regulatory gene involved in acetate induction of aciA and amdS expression. The function of the aciA gene is not yet known.
amdA基因是控制构巢曲霉中amdS基因表达的多个反式作用调节基因之一。已发现一种分子量约为42,000的多肽在amdA突变菌株中组成型合成且可被乙酸盐诱导。使用含cDNA探针的差异筛选分离出了一个λ克隆，该克隆含有一个名为aciA的基因，此基因编码该多肽。通过用含aciA的探针探测Northern印迹，已鉴定出两种乙酸盐诱导的RNA物种。有人提出amdA基因是一个参与aciA和amdS表达的乙酸盐诱导的调节基因。aciA基因的功能尚不清楚。
