Peeters M, Janssens W, Fransen K, Brandful J, Heyndrickx L, Koffi K, Delaporte E, Piot P, Gershy-Damet G M, van der Groen G
Department of Infection and Immunity, Institute of Tropical Medicine, Antwerp, Belgium.
AIDS Res Hum Retroviruses. 1994 Oct;10(10):1289-94. doi: 10.1089/aid.1994.10.1289.
To search for the presence of SIV in sooty mangabeys and other monkey species in Côte d'Ivoire, West Africa, and to compare viral isolates with HIV-2 strains from the same region.
Forty-three captive housed monkeys (28 African green monkeys, 6 sooty mangabeys, 6 baboons, and 6 patas monkeys) were tested for the presence of HIV and SIV antibodies. Virus was isolated from the peripheral blood lymphocytes of seropositive animals and from HIV-2 antibody-positive patients originating from Côte d'Ivoire, Ghana, Senegal, and Belgium. Viruses were characterized by Western blot and radioimmunoprecipitation assay. Proviral DNA was amplified by PCR, cloned, and sequenced to construct a phylogenetic tree.
One African green monkey and three sooty mangabeys had antibodies that cross-reacted with HIV-2. From two mangabeys lentiviruses were isolated and designated as SIVsmCI2 and SIVsmCI8. Serological, virological, and sequence data showed that these isolates are members of the HIV-2/SIVsm/SIVmac group of primate lentiviruses. Furthermore, in the phylogenetic tree, these two new viruses form a distinct subgroup that is equidistant to the HIV-2 strains and the previously described SIVsm/SIVmac viruses.
This study provides additional evidence that sooty mangabey monkeys can be infected with a lentivirus in their natural habitat. Within the SIVsm and SIVmac viruses extensive genetic variation is observed.
在西非科特迪瓦的乌黑白眉猴及其他猴类物种中寻找猴免疫缺陷病毒（SIV）的存在，并将病毒分离株与来自同一地区的HIV - 2毒株进行比较。
对43只圈养猴子（28只非洲绿猴、6只乌黑白眉猴、6只狒狒和6只赤猴）进行HIV和SIV抗体检测。从血清阳性动物的外周血淋巴细胞以及来自科特迪瓦、加纳、塞内加尔和比利时的HIV - 2抗体阳性患者中分离病毒。通过蛋白质印迹法和放射免疫沉淀试验对病毒进行鉴定。通过聚合酶链反应（PCR）扩增前病毒DNA，进行克隆和测序以构建系统发育树。
一只非洲绿猴和三只乌黑白眉猴具有与HIV - 2发生交叉反应的抗体。从两只白眉猴中分离出慢病毒，分别命名为SIVsmCI2和SIVsmCI8。血清学、病毒学和序列数据表明，这些分离株是灵长类慢病毒HIV - 2/SIVsm/SIVmac组的成员。此外，在系统发育树中，这两种新病毒形成一个独特的亚组，与HIV - 2毒株和先前描述的SIVsm/SIVmac病毒距离相等。
本研究提供了额外证据，证明乌黑白眉猴在其自然栖息地可感染慢病毒。在SIVsm和SIVmac病毒中观察到广泛的基因变异。
