冈比亚一个村庄沙眼的分子流行病学
Molecular epidemiology of trachoma in a Gambian village.
作者信息
Bailey R L, Hayes L, Pickett M, Whittle H C, Ward M E, Mabey D C
机构信息
Department of Clinical Sciences, London School of Hygiene and Tropical Medicine.
出版信息
Br J Ophthalmol. 1994 Nov;78(11):813-7. doi: 10.1136/bjo.78.11.813.
Abstract
The application of a diagnostic and genotyping technique based on the polymerase chain reaction (PCR) to the study of trachoma epidemiology in the Gambian village of Jali is reported. PCR based on the major outer membrane protein (MOMP) gene of Chlamydia trachomatis appears to be more sensitive than either isolation or antigen detection by enzyme immunoassay; it had a specificity of 95% and sensitivity of 51% against clinical signs. PCR genotyping identified genotypes A and B of Chlamydia trachomatis circulating in Jali. Sequencing revealed a Pst1 restriction endonuclease site in the amplified MOMP gene of some B strains but not others; Pst1 digestion of the PCR product proved an easy method of distinguishing these strains. The distribution of serotypes and B strain variants shows a significant degree of household clustering (p < 0.001). PCR based genotyping combined with strain typing provides a new and powerful epidemiological tool for the study of transmission events in trachoma.
摘要
报告了一种基于聚合酶链反应（PCR）的诊断和基因分型技术在冈比亚贾利村沙眼流行病学研究中的应用。基于沙眼衣原体主要外膜蛋白（MOMP）基因的PCR似乎比酶免疫测定法进行分离或抗原检测更为敏感；针对临床症状，其特异性为95%，敏感性为51%。PCR基因分型确定了在贾利村流行的沙眼衣原体A和B基因型。测序显示，部分B菌株的扩增MOMP基因中有Pst1限制性内切酶位点，而其他菌株则没有；PCR产物的Pst1消化证明是区分这些菌株的简便方法。血清型和B菌株变体的分布显示出显著的家庭聚集程度（p<0.001）。基于PCR的基因分型与菌株分型相结合，为沙眼传播事件的研究提供了一种新的强大的流行病学工具。
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