Liochev S I, Fridovich I
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA.
Arch Biochem Biophys. 1995 Apr 20;318(2):408-10. doi: 10.1006/abbi.1995.1247.
Glucose oxidase reduces nitroblue tetrazolium, or ferricytochrome c, faster anaerobically than aerobically. This result is inconsistent with the conclusion that GO2 can reduce O2 to O2- which is then responsible for the reduction of NBT and of cytochrome c. Nevertheless, the aerobic reductions are partially inhibitable by superoxide dismutase. A scheme of reactions is proposed which explains why these electron acceptors cause an O2- production which does not occur in their absence when O2 is the sole electron acceptor.
葡萄糖氧化酶在厌氧条件下比需氧条件下能更快地还原硝基蓝四氮唑或铁细胞色素c。这一结果与“GO2能将O2还原为O2-，然后O2-负责还原NBT和细胞色素c”这一结论不一致。然而，需氧还原反应可被超氧化物歧化酶部分抑制。本文提出了一个反应方案，解释了为什么这些电子受体在以O2为唯一电子受体时不存在的情况下会产生O2-。
