Hannuksela S, Tenovuo J, Roger V, Lenander-Lumikari M, Ekstrand J
Institute of Dentistry, University of Turku, Finland.
Caries Res. 1994;28(6):429-34. doi: 10.1159/000262016.
Fluoride (F-) ions at concentrations present in vivo at the plaque/enamel interface (0.05-10 mM) inhibited the activities of lactoperoxidase (LP), myeloperoxidase (MP) and total salivary peroxidase (TSP) in a pH- and dose-dependent way. The inhibition was observed only at pH < or = 6.5 and with F- concentrations > or = 0.1 mM. At pH 5.5 LP activity was inhibited by 85% and MP by 34% with 10 mM F-. TSP activity was also inhibited only at low pH (5.5) by approximately 25%. Furthermore, the generation of the actual antimicrobial agent in vivo, hypothiocyanite (HOSCN/OSCN-), of the oral peroxidase systems was inhibited by F-, again at low pH (5.0-5.5) both in buffer (by 45%) and in saliva (by 15%). This inhibition was observed only with the highest F- concentrations studied (5-10 mM). Fluoridated toothpaste (with 0.10 or 0.14% F) mixed with saliva did not inhibit TSP or HOSCN/OSCN- generation. This may have been due to the 'buffering' effect of toothpaste which did not allow salivary pH to drop below 5.9. We conclude that the F- ions in acidic fluoride products, e.g. in gels or varnishes (but not in toothpastes), may have the potential to locally inhibit the generation of a nonimmune host defense factor, HOSCN/OSCN/SCN-, produced by oral peroxidase systems. The possible clinical significance of this finding remains to be shown.
菌斑/牙釉质界面处体内存在的浓度（0.05 - 10 mM）的氟离子（F-）以pH和剂量依赖的方式抑制了乳过氧化物酶（LP）、髓过氧化物酶（MP）和总唾液过氧化物酶（TSP）的活性。仅在pH≤6.5且F-浓度≥0.1 mM时观察到这种抑制作用。在pH 5.5时，10 mM F-使LP活性抑制85%，MP活性抑制34%。TSP活性也仅在低pH（5.5）时被抑制约25%。此外，口腔过氧化物酶系统在体内实际抗菌剂次硫氰酸盐（HOSCN/OSCN-）的生成在低pH（5.0 - 5.5）时受到F-的抑制，在缓冲液中（抑制45%）和唾液中（抑制15%）均如此。仅在研究的最高F-浓度（5 - 10 mM）下观察到这种抑制作用。与唾液混合的含氟牙膏（含0.10%或0.14% F）不会抑制TSP或HOSCN/OSCN-的生成。这可能是由于牙膏的“缓冲”作用，使唾液pH不会降至5.9以下。我们得出结论，酸性氟化物产品中的F-离子，例如凝胶或清漆中的（但牙膏中不是），可能有潜力局部抑制口腔过氧化物酶系统产生的非免疫宿主防御因子HOSCN/OSCN/SCN-。这一发现的可能临床意义仍有待证明。
