Shimmen T, Mimura T, Kikuyama M, Tazawa M
Department of Life Science, Faculty of Science, Himeji Institute of Technology, Hyogo, Japan.
Cell Struct Funct. 1994 Oct;19(5):263-78. doi: 10.1247/csf.19.263.
Characean cells have contributed significantly to various areas of plant cell biology such as cell motility and membrane transport. Since characean cells are very large, various kinds of operations can easily be applied to them. Development of techniques of intracellular perfusion and permeabilization of plasma membrane has facilitated studies on functions of the plasma membrane and the vacuolar membrane (or tonoplast) which is specific to plant cells. The present article is aimed at reviewing the contribution of characean cells to the study of electrophysiological characteristics of plant membranes. Our attention was mainly focused on experiments using plasma membrane-permeabilized cells and intracellularly perfused cells.
