Busch G J, Schamberg J F, Moretz R C, Strom T B, Tilney N L, Carpenter C B
Lab Invest. 1976 Sep;35(3):272-80.
To define the composition and proliferative and functional activity of the rejection formed sheep erythrocyte rosettes (T cells), expressed surface Ig (B cells) or Fc receptors and specific lymphocyte-mediated cytotoxic activity. Small clumped lymphocytes (48.8 +/- 18.9 per cent) and plasma cells (18.8 +/- 10.9 per cent) were most common and correlated negatively (p less than 0.01). Percentages of surface Ig-bearing and small clumped lymphocytes correlated directly (p less than 0.05) as did the percentages of E rosette-forming and plasma cells (p less than 0.05). The percentages of surface Ig-bearing B cells correlated negatively with E rosette-forming T cells (p less than 0.05). Within this spectrum of rejection, three patterns could be identified. In the first pattern, humoral rejection (by immunofluorescence) was intense and cellular infiltration was primarily by surface Ig and Fc receptor-positive small clumped B lymphocytes. The second and most common pattern consisted of a mixed T and B cell infiltrate with intermediate to high lymphocyte-mediated cytotoxic activity and variable humoral rejection. In the third pattern, invitro studies showed primarily E rosette-forming T cells with paradoxically low lymphocyte-mediated cytotoxic activity. However, morphologic studies also revealed the highest percentages of plasma cells but only mild humoral rejection. Thus, combined morphologic and in vitro studies identified three patterns of protracted renal allograft rejection. Thus, combined morphologic and in vitro studies identified three patterns of protracted renal allograft rejection for which the percentages of plasma cells may provide a distinguishing morphologic marker. Furthermore, the degree of lymphocytic invasion of renal tubules and blood vessels may provide an estimate of lymphocyte-mediated cytotoxic activity.
为确定形成绵羊红细胞玫瑰花结的（T细胞）、表达表面免疫球蛋白（B细胞）或Fc受体以及特异性淋巴细胞介导的细胞毒活性的排斥反应的组成、增殖和功能活性。小团块状淋巴细胞（48.8±18.9%）和浆细胞（18.8±10.9%）最为常见，且呈负相关（p<0.01）。带有表面免疫球蛋白的淋巴细胞和小团块状淋巴细胞的百分比呈正相关（p<0.05），形成E玫瑰花结的细胞和浆细胞的百分比也呈正相关（p<0.05）。带有表面免疫球蛋白的B细胞百分比与形成E玫瑰花结的T细胞呈负相关（p<0.05）。在这种排斥反应范围内，可识别出三种模式。在第一种模式中，体液排斥反应（通过免疫荧光法）强烈，细胞浸润主要由表面免疫球蛋白和Fc受体阳性的小团块状B淋巴细胞引起。第二种也是最常见的模式是T和B细胞混合浸润，具有中等至高的淋巴细胞介导的细胞毒活性和可变的体液排斥反应。在第三种模式中，体外研究显示主要是形成E玫瑰花结的T细胞，其淋巴细胞介导的细胞毒活性异常低。然而，形态学研究也显示浆细胞的百分比最高，但体液排斥反应仅为轻度。因此，形态学和体外研究相结合确定了三种长期肾移植排斥反应模式。因此，形态学和体外研究相结合确定了三种长期肾移植排斥反应模式，浆细胞的百分比可能为其提供一个可区分的形态学标志。此外，肾小管和血管的淋巴细胞浸润程度可提供淋巴细胞介导的细胞毒活性的估计值。
