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噬菌体λ转导衍生物的体外构建

The construction in vitro of transducing derivatives of phage lambda.

作者信息

Borck K, Beggs J D, Brammar W J, Hopkins A S, Murray N E

出版信息

Mol Gen Genet. 1976 Jul 23;146(2):199-207. doi: 10.1007/BF00268089.

DOI:10.1007/BF00268089
PMID:785220
Abstract

Methods are described for the construction of plaque-forming, transducing derivatives of phage lambda, using appropirate receptor genomes and fragments of DNA generated by the restriction enzymes endo R.EcoRI and endo R.HindIII. The general properties of the transducing derivatives are described and discussed. Plaque-forming phages carrying the E. coli trp, his, cysB, thyA, supD, supE, sup F, hsd, tna and lig genes have been isolated.

摘要

本文描述了构建噬菌体λ的噬菌斑形成转导衍生物的方法，使用合适的受体基因组以及由限制性内切酶EcoRI和HindIII产生的DNA片段。对转导衍生物的一般特性进行了描述和讨论。已分离出携带大肠杆菌trp、his、cysB、thyA、supD、supE、supF、hsd、tna和lig基因的噬菌斑形成噬菌体。

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The construction in vitro of transducing derivatives of phage lambda.噬菌体λ转导衍生物的体外构建
Mol Gen Genet. 1976 Jul 23;146(2):199-207. doi: 10.1007/BF00268089.
2
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J Mol Biol. 1975 Nov 5;98(3):551-64. doi: 10.1016/s0022-2836(75)80086-6.
4
[Transducing lambda phages with Escherichia coli genes].[用大肠杆菌基因转导λ噬菌体]
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The isolation and genetic characteristics of lambda transducing phages of the uvrA+ and uvrC+ genes of E. coli K12.大肠杆菌K12的uvrA +和uvrC +基因的λ转导噬菌体的分离及遗传特性
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Specialized transducing phages for ribosomal protein genes of Escherichia coli.用于大肠杆菌核糖体蛋白基因的特异性转导噬菌体。
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Segregation of New Lysogenic Types during Growth of a Doubly Lysogenic Strain Derived from Escherichia Coli K12.源于大肠杆菌K12的双重溶源菌株生长过程中新溶源类型的分离
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Fusions of the lac and trp Regions of the Escherichia coli Chromosome.大肠杆菌染色体中乳糖操纵子和色氨酸操纵子区域的融合
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Bacterial delta-aminolevulinic acid synthase activity is not essential for leghemoglobin formation in the soybean/Bradyrhizobium japonicum symbiosis.在大豆/根瘤菌共生体中，细菌 δ-氨基乙酰丙酸合酶活性对于豆血红蛋白的形成不是必需的。
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Gene cluster responsible for validamycin biosynthesis in Streptomyces hygroscopicus subsp. jinggangensis 5008.井冈霉素产生菌吸水链霉菌井冈变种5008中负责井冈霉素生物合成的基因簇
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Streptomyces coelicolor A3(2) lacks a genomic island present in the chromosome of Streptomyces lividans 66.天蓝色链霉菌A3(2)缺乏存在于淡青链霉菌66染色体中的一个基因组岛。
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Tracking EcoKI and DNA fifty years on: a golden story full of surprises.五十年后的EcoKI与DNA追踪：一个充满惊喜的精彩故事。
Nucleic Acids Res. 2003 Dec 15;31(24):7059-69. doi: 10.1093/nar/gkg944.
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Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae.利用高通量基因破坏系统在肺炎链球菌中鉴定113个保守必需基因。
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The production of phage chromosome fragments and their capacity for genetic transfer.噬菌体染色体片段的产生及其基因转移能力。
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Transduction and recombination study of linkage relationships among the genes controlling tryptophan synthesis in Escherichia coli.大肠杆菌中控制色氨酸合成的基因间连锁关系的转导与重组研究。
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The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage lambda-dg.用从噬菌体λ-dg分离的脱氧核糖核酸对大肠杆菌进行转化。
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Mutation to extended host range and the occurrence of phenotypic mixing in the temperate coliphage lambda.温和型大肠杆菌噬菌体λ的宿主范围扩展突变及表型混合的发生
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