噬菌体λ转导衍生物的体外构建
The construction in vitro of transducing derivatives of phage lambda.
作者信息
Borck K, Beggs J D, Brammar W J, Hopkins A S, Murray N E
出版信息
Mol Gen Genet. 1976 Jul 23;146(2):199-207. doi: 10.1007/BF00268089.
PMID:785220
Abstract
Methods are described for the construction of plaque-forming, transducing derivatives of phage lambda, using appropirate receptor genomes and fragments of DNA generated by the restriction enzymes endo R.EcoRI and endo R.HindIII. The general properties of the transducing derivatives are described and discussed. Plaque-forming phages carrying the E. coli trp, his, cysB, thyA, supD, supE, sup F, hsd, tna and lig genes have been isolated.
摘要
本文描述了构建噬菌体λ的噬菌斑形成转导衍生物的方法，使用合适的受体基因组以及由限制性内切酶EcoRI和HindIII产生的DNA片段。对转导衍生物的一般特性进行了描述和讨论。已分离出携带大肠杆菌trp、his、cysB、thyA、supD、supE、supF、hsd、tna和lig基因的噬菌斑形成噬菌体。
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