用限制性核酸内切酶EcoRI、SmaI和HindIII对携带大肠杆菌K-12的argA、argECBH、argF和argI操纵子的λ和φ80转导噬菌体进行DNA切割。

DNA cleavage of lambda and phi 80 transducing phages carrying the argA, argECBH, argF and argI operons of Escherichia coli K-12 with the restriction endonucleases EcoRI, SmaI and HindIII.

作者信息

Moore S K, James E

出版信息

Gene. 1978 Feb;3(1):53-80. doi: 10.1016/0378-1119(78)90007-0.

Abstract

DNA isolated from each of the seven arginine transducing phages lambdaargA2cI857susS7, phi80ppc argECBH, phi80argF, phi80argF ilambdacI857, lambdaargF2, lambdaargF23 and lambdaargI valScI857susS7 has been specifically cleaved by the restriction endonucleases EcoRI, SmaI and HindIII. The DNA fragments resulting from single, and in some cases, double endonuclease digests were separated by electrophoresis in agarose and also in polyacrylamide gel. The electrophoretic patterns thus obtained were compared with those produced by digestion of DNA isolated from the corresponding lambda and phi80 parental phages. The majority of cleavage sites produced by the action of these restriction enzymes on arginine transducing DNA have been physically mapped.

摘要

从七种精氨酸转导噬菌体λargA2cI857susS7、φ80ppc argECBH、φ80argF、φ80argF ilambdacI857、λargF2、λargF23和λargI valScI857susS7中分离出的DNA已被限制性内切酶EcoRI、SmaI和HindIII特异性切割。单酶切以及某些情况下的双酶切产生的DNA片段通过琼脂糖凝胶电泳和聚丙烯酰胺凝胶电泳进行分离。将由此获得的电泳图谱与从相应的λ和φ80亲本噬菌体中分离出的DNA消化产生的图谱进行比较。这些限制酶作用于精氨酸转导DNA产生的大多数切割位点已进行了物理定位。