Wilks M, Tabaqchali S
Department of Medical Microbiology, St Bartholomew's Hospital Medical College, West Smithfield, London, UK.
J Hosp Infect. 1994 Nov;28(3):231-4. doi: 10.1016/0195-6701(94)90105-8.
We assessed the use of the polymerase chain reactions (PCR) with an arbitrary primer (AP-PCR) to investigate a major hospital outbreak of diarrhoea due to Clostridium difficile. A single pattern consisting of three bands of 240, 580 and 1100 bp was obtained from all isolates studied. AP-PCR is a simple, rapid technique which should find increased application in the rapid investigation of suspected outbreaks of many different bacterial species, particularly new pathogens or those for which no accepted typing scheme exists.
我们评估了使用带有任意引物的聚合酶链反应（AP-PCR）来调查一家大型医院因艰难梭菌引起的腹泻暴发情况。从所有研究的分离株中获得了一个由240、580和1100 bp三条带组成的单一图谱。AP-PCR是一种简单、快速的技术，在快速调查许多不同细菌物种的疑似暴发中，尤其是新病原体或那些尚无公认分型方案的病原体时，其应用有望增加。
