Janezic Sandra
National Laboratory for Health, Environment and Food (NLZOH), Prvomajska 1, 2000, Maribor, Slovenia.
Faculty of Medicine, University of Maribor, Taborska ulica 8, 2000, Maribor, Slovenia.
Methods Mol Biol. 2016;1476:15-21. doi: 10.1007/978-1-4939-6361-4_2.
PCR-ribotyping, a method based on heterogeneity of ribosomal intergenic spacer region, is the preferred method for genotyping of Clostridium difficile. Standardly used procedure for PCR-ribotyping is culturing of C. difficile from fecal samples and subsequent typing. In this chapter, we describe a modified PCR-ribotyping method for direct detection of PCR-ribotypes directly in total stool DNA extract, without prior need to isolate C. difficile.
PCR核糖体分型是一种基于核糖体基因间隔区异质性的方法，是艰难梭菌基因分型的首选方法。PCR核糖体分型的标准使用程序是从粪便样本中培养艰难梭菌并随后进行分型。在本章中，我们描述了一种改良的PCR核糖体分型方法，可直接在粪便总DNA提取物中直接检测PCR核糖体类型，而无需事先分离艰难梭菌。
