Pintó R M, Diez J M, Bosch A
Department of Microbiology, University of Barcelona, Spain.
J Med Virol. 1994 Nov;44(3):310-5. doi: 10.1002/jmv.1890440317.
The use of the continuous cell line CaCo-2 as an in vivo amplification system for the detection of fastidious human enteric viruses is reported. CaCo-2 cells showed an increased sensitivity to laboratory strains of group A rotavirus 3, reovirus 3, astrovirus 1, poliovirus 1, coxsackievirus A 24, enterovirus 70, and adenovirus 5, 40 and 41, when compared to a routine host cell line for each virus. Nucleic acids from wild-type infectious rotavirus, astrovirus, and adenovirus 40 in stool samples of patients with acute gastroenteritis could be amplified after infection of CaCo-2 cells with trypsin-pre-treated virus inocula. Virus diagnosis was carried out subsequently by dot-blot hybridisation with specific cDNA probes. An amplification factor between 10 and 1,000x was obtained by infection of CaCo-2 cells, thus enabling specific detection of low numbers of a wide range of enteric viruses, and the differentiation between infectious and noninfectious particles.
据报道，连续细胞系CaCo-2可作为体内扩增系统用于检测苛求的人类肠道病毒。与每种病毒的常规宿主细胞系相比，CaCo-2细胞对A组轮状病毒3型、呼肠孤病毒3型、星状病毒1型、脊髓灰质炎病毒1型、柯萨奇病毒A24型、肠道病毒70型以及腺病毒5型、40型和41型的实验室毒株表现出更高的敏感性。用胰蛋白酶预处理的病毒接种物感染CaCo-2细胞后，急性胃肠炎患者粪便样本中野生型感染性轮状病毒、星状病毒和腺病毒40的核酸能够得到扩增。随后通过与特异性cDNA探针进行斑点杂交来进行病毒诊断。通过感染CaCo-2细胞获得了10至1000倍的扩增因子，从而能够特异性检测多种肠道病毒的低数量病毒，并区分感染性和非感染性颗粒。
