D'Souza-Schorey C, Li G, Colombo M I, Stahl P D
Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO 63110.
Science. 1995 Feb 24;267(5201):1175-8. doi: 10.1126/science.7855600.
Adenosine diphosphate-ribosylation factor 6 (ARF6), ARF6 mutants, and ARF1 were transiently expressed in Chinese hamster ovary cells, and the effects on receptor-mediated endocytosis were assessed. Overexpressed ARF6 localized to the cell periphery and led to a redistribution of transferrin receptors to the cell surface and a decrease in the rate of uptake of transferrin. Similar results were obtained when a mutant defective in guanosine triphosphate hydrolysis was expressed. Expression of a dominant negative mutant, ARF6(T27N), resulted in an intracellular distribution of transferrin receptors and an inhibition of transferrin recycling to the cell surface. In contrast, overexpression of ARF1 had little or no effect on these parameters of endocytosis.
二磷酸腺苷核糖基化因子6（ARF6）、ARF6突变体和ARF1在中国仓鼠卵巢细胞中瞬时表达，并评估其对受体介导的内吞作用的影响。过表达的ARF6定位于细胞周边，导致转铁蛋白受体重新分布到细胞表面，并降低转铁蛋白的摄取速率。当表达一种在鸟苷三磷酸水解方面有缺陷的突变体时，也获得了类似的结果。显性负性突变体ARF6（T27N）的表达导致转铁蛋白受体在细胞内分布，并抑制转铁蛋白循环回到细胞表面。相比之下，ARF1的过表达对这些内吞作用参数几乎没有影响。
