Wuyts N, Chokesajjawatee N, Panyim S
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 1994 Jun;25(2):266-71.
In Southeast Asia Trypanosoma evansi infection is a disease of economic importance since it affects the health of buffalo, cattle and swine. The acute stage symptoms include abortion, central nervous system disorder and even death, and in the chronic condition working capacity and productivity of the animals are affected. A polymerase chain reaction (PCR)-based detection technique has been developed with a sensitivity of 0.5 pg of parasite DNA or one single parasite in 10 microliters of blood samples which were allowed to clot and then boiled before DNA amplification. This permitted storage of blood collection at ambient temperature for at least one month. Phosphate-saline-glucose solution, normally used as trypanosome maintenance buffer, inhibited PCR. Although DNA primers used were derived from T. evansi specific sequence, amplification of the genome of T. brucei and T. equiperdum generated the same 227 bp fragment. This method should now make it possible to detect infections in livestock in the very early stages where microscope examination is equivocal and to monitor groups of animals after trypanocidal treatment.
在东南亚，伊氏锥虫感染是一种具有经济重要性的疾病，因为它会影响水牛、牛和猪的健康。急性期症状包括流产、中枢神经系统紊乱甚至死亡，而在慢性期，动物的工作能力和生产力会受到影响。已经开发出一种基于聚合酶链反应（PCR）的检测技术，其灵敏度为0.5皮克寄生虫DNA或10微升血液样本中的单个寄生虫，血液样本在DNA扩增前先凝固然后煮沸。这使得血液采集样本能够在室温下保存至少一个月。通常用作锥虫维持缓冲液的磷酸盐 - 盐水 - 葡萄糖溶液会抑制PCR。尽管所用的DNA引物源自伊氏锥虫特异性序列，但布氏锥虫和马媾疫锥虫基因组的扩增产生了相同的227 bp片段。现在，这种方法应该能够在显微镜检查结果不明确的早期阶段检测家畜感染，并在进行杀锥虫治疗后监测动物群体。
