Ficek W
Department of Animal Physiology, Institute of Zoology, Jagiellonian University, Cracow, Poland.
Toxicol Ind Health. 1994 May-Jun;10(3):191-201. doi: 10.1177/074823379401000308.
This experiment was performed to investigate changes in the thymus of Wistar-strain male rats administered cadmium chloride (CdCl2), mercuric chloride (HgCl2), and lead chloride (PbCl2) salts. The study involved measurement of: 1) deoxyribonucleic acid (DNA) content; 2) ascorbic acid (vitamin C) content; 3) incorporation of 3h-thymidine during in-vitro investigation; and 4) histological and morphological changes. It was shown in investigations on DNA content that administration of heavy metal salts caused an average 76% decrease in DNA content compared to the control. Moreover, in the thymus of experimental animals, an average 69% decrease in vitamin C content was recorded. In in vitro cultures, heavy metal salts decreased the incorporation of 3H-thymidine in thymic cells.
进行该实验是为了研究给予氯化镉（CdCl2）、氯化汞（HgCl2）和氯化铅（PbCl2）盐的Wistar品系雄性大鼠胸腺的变化。该研究包括测量：1）脱氧核糖核酸（DNA）含量；2）抗坏血酸（维生素C）含量；3）体外研究期间3h-胸腺嘧啶核苷的掺入；以及4）组织学和形态学变化。关于DNA含量的研究表明，与对照组相比，给予重金属盐导致DNA含量平均下降76%。此外，在实验动物的胸腺中，维生素C含量平均下降69%。在体外培养中，重金属盐减少了胸腺细胞中3H-胸腺嘧啶核苷的掺入。
