Woldehiwet Z, Hussin A A
University of Liverpool, Department of Veterinary Pathology, Neston, Wirral, UK.
Vet Immunol Immunopathol. 1994 Nov;43(4):389-400. doi: 10.1016/0165-2427(94)90159-7.
Ten lambs were experimentally infected with Border disease virus and the distribution of viral antigen in lymphocyte subpopulations studied by flow cytometry. The virus was isolated in culture from the peripheral blood mononuclear cells (MNC) of all experimentally infected lambs for a mean period of 9.8 +/- 1.03 days. The peak virus titre of 3.26 log10 TCID50 per 10(6) MNC was attained Day 9 post-inoculation (pi). Viral antigen was present in peripheral blood lymphocytes of experimentally infected lambs as early as 24 h pi and continued to be detected up to Day 10 pi. The number of lymphocytes expressing viral antigen rose from 12.38 +/- 1.22% in samples taken Day 3 pi to 23.21 +/- 2.82% on those collected Day 7 pi, dropping gradually thereafter. During the peak period of infection, 12.46 +/- 2.09% of B cells, 37.71 +/- 10.96% of T cells and 52.33 +/- 8.27% of lymphocytes which were neither B nor T lymphocytes expressed viral antigen. Most of the infected lymphocytes expressed the OvCD5 (T cell) molecule. The virus affected all T cell subsets but the suppressor/cytotoxic (OvCD8+) cells appeared to be the main targets. During the peak period of infection, 54.20 +/- 6.16% of the infected T cells expressed the OvCD8 molecule, 31.58 +/- 7.12% were OvCD4+, and 12.67 +/- 6.50% were OvWC1+ (T-19+, gamma/delta).
选用10只羔羊进行实验性边境病病毒感染，并通过流式细胞术研究病毒抗原在淋巴细胞亚群中的分布。从所有实验感染羔羊的外周血单个核细胞（MNC）中培养分离出病毒，平均持续时间为9.8±1.03天。接种后第9天（pi），每10⁶个MNC的病毒滴度峰值达到3.26 log₁₀ TCID₅₀。早在接种后24小时，实验感染羔羊的外周血淋巴细胞中就出现了病毒抗原，并持续检测到接种后第10天。表达病毒抗原的淋巴细胞数量从接种后第3天采集样本中的12.38±1.22%上升至接种后第7天采集样本中的23.21±2.82%，此后逐渐下降。在感染高峰期，12.46±2.09%的B细胞、37.71±10.96%的T细胞以及52.33±8.27%的非B非T淋巴细胞表达病毒抗原。大多数受感染的淋巴细胞表达OvCD5（T细胞）分子。该病毒影响所有T细胞亚群，但抑制性/细胞毒性（OvCD8⁺）细胞似乎是主要靶标。在感染高峰期，54.20±6.16%的受感染T细胞表达OvCD8分子，31.58±7.12%为OvCD4⁺，12.67±6.50%为OvWC1⁺（T-19⁺，γ/δ）。
