Woldehiwet Z, Hussin A A
University of Liverpool, Department of Veterinary Pathology, Leahurst, Neston, Wirral, UK.
Vet Immunol Immunopathol. 1994 Jun;41(3-4):201-9. doi: 10.1016/0165-2427(94)90097-3.
Mononuclear cells cytotoxic to a noncytopathic strain of Border disease virus (BDV) but not to uninfected cells were detected in the peripheral blood of experimentally infected lambs, 10 days after experimental infection, reaching peak levels of cytotoxicity 15 days post-inoculation. The specificity of the cytotoxic activity to BDV-infected cells was tested by including autologous targets infected with another virus (bovine respiratory syncytial virus, BRSV) and normal uninfected autologous targets. The cytotoxic T cells were virus-specific, as only autologous BDV-infected targets were lysed, whereas autologous targets infected with BRSV and uninfected targets were not lysed. The present results also suggest that the cytotoxic activity was largely MHC-restricted as the specific release from BDV-infected autologous targets was significantly higher than that of BDV-infected heterologous targets (P < 0.001) and the cytotoxicity against BDV-infected homologous targets was significantly reduced by the selective depletion of OvCD8+ T cells (P < 0.001).
在实验感染的羔羊外周血中，于实验感染后10天检测到对边境病病毒（BDV）非细胞病变株具有细胞毒性但对未感染细胞无细胞毒性的单核细胞，接种后15天细胞毒性达到峰值水平。通过加入感染另一种病毒（牛呼吸道合胞病毒，BRSV）的自体靶细胞和正常未感染的自体靶细胞，测试了对BDV感染细胞的细胞毒性活性的特异性。细胞毒性T细胞具有病毒特异性，因为仅自体BDV感染的靶细胞被裂解，而感染BRSV的自体靶细胞和未感染的靶细胞未被裂解。目前的结果还表明，细胞毒性活性在很大程度上受主要组织相容性复合体（MHC）限制，因为BDV感染的自体靶细胞的特异性释放显著高于BDV感染的异源靶细胞（P < 0.001），并且通过选择性清除绵羊CD8 + T细胞，对BDV感染的同源靶细胞的细胞毒性显著降低（P < 0.001）。
