Woldehiwet Z, Hussin A A
University of Liverpool, Department of Veterinary Pathology, Neston, Wirral, UK.
Vet Immunol Immunopathol. 1994 Aug;42(2):127-35. doi: 10.1016/0165-2427(94)90002-7.
Biotinylated virus-specific antibodies were used to detect Border disease virus antigen by flow cytometry in the mononuclear cells of the peripheral blood of 15 sheep persistently infected with Border disease virus. The viral antigen was present in 12.86 +/- 4.65% (mean +/- SD) of mononuclear cells (MNC). The percentage of MNC that contained viral antigen was higher in lambs than in adult sheep, with mean rates of 22.68 +/- 5.02% and 11.65 +/- 4.39%, respectively. Depletion methods were used to estimate the distribution of viral antigen in peripheral blood lymphocyte subsets. The viral antigen was present in T-cells (OvCD5+), B-cells (LCAp220+) and non-T- and non-B-cells. Depletion studies revealed that 5.39 +/- 2.47% of the cells expressing the LCAp220 epitope (B-cells), 23.38 +/- 11.38% of those expressing the OvCD5 epitope (T-cells) and 55.07 +/- 10.93% of those which were neither B- nor T-cells were positive for viral antigen. Most (57.18 +/- 5.41%) of the T-cells containing viral antigen were cytotoxic/suppressor (OvCD8), 25.63 +/- 2.97% were helper (OvCD4) cells and 12.24 +/- 3.21% expressed the gamma/delta (OvWC1) epitope.
使用生物素化的病毒特异性抗体，通过流式细胞术检测15只持续感染边界病病毒的绵羊外周血单个核细胞中的边界病病毒抗原。病毒抗原存在于12.86±4.65%（平均值±标准差）的单个核细胞（MNC）中。含有病毒抗原的MNC百分比在羔羊中高于成年绵羊，平均比例分别为22.68±5.02%和11.65±4.39%。采用去除法估计病毒抗原在外周血淋巴细胞亚群中的分布。病毒抗原存在于T细胞（OvCD5+）、B细胞（LCAp220+）以及非T非B细胞中。去除研究显示，表达LCAp220表位的细胞（B细胞）中有5.39±2.47%、表达OvCD5表位的细胞（T细胞）中有23.38±11.38%以及既非B细胞也非T细胞的细胞中有55.07±10.93%的细胞病毒抗原呈阳性。含有病毒抗原的T细胞中，大多数（57.18±5.41%）是细胞毒性/抑制性（OvCD8）细胞，25.63±2.97%是辅助性（OvCD4）细胞，12.24±3.21%表达γ/δ（OvWC1）表位。
