Maillard N, Wolczynski S, Argyriou A, Drosdowsky M A, Foucault P, Carreau S
Medical Academy-Bialystok-Pologne, Biochimie-CHU, Biochimie-IRBA, ER CNRS 90, Université de Caen, France.
Arch Androl. 1994 Nov-Dec;33(3):187-99. doi: 10.3109/01485019408987823.
In two different enriched populations of Leydig cells (called FI and FII) obtained from human testes (young patients: mean age 36 +/- 3 years, n = 6; aged men: mean age 73 +/- 2 years, n = 5), the dehydroepiandrosterone and testosterone in vitro outputs were increased in a dose- and time-related manners by hCG. Similar results were obtained when the Leydig cells were incubated in presence of either dbcAMP or 22R-hydroxycholesterol. In presence of either hCG or dbcAMP, the coefficient of stimulation (in terms of steroid outputs) was higher in FII when compared to FI. Conversely, the basal production of steroids was greater in FI than in FII, mainly for testosterone. The addition of increasing amounts of seminiferous tubule culture medium (STM) to the Leydig cell incubation medium led to a dose-related enhancement of the steroid production in both enriched-Leydig cell fractions under basal and hCG-stimulated conditions. Similar results were obtained in presence of increased seminiferous tubules length. Additional experiments realized with either concentrated STM or the coculture of seminiferous tubules with purified Leydig cells have confirmed the existence of a paracrine control of Leydig cell steroidogenesis by seminiferous secreted factor(s). A paracrine factor (or factors) from seminiferous tubular origin influences positively and with a high efficiency the Leydig cell function in humans, whatever the age.
在从人类睾丸获取的两种不同的富集莱迪希细胞群体（称为FI和FII）中（年轻患者：平均年龄36±3岁，n = 6；老年男性：平均年龄73±2岁，n = 5），人绒毛膜促性腺激素（hCG）以剂量和时间相关的方式增加了脱氢表雄酮和睾酮的体外产量。当莱迪希细胞在二丁酰环磷腺苷（dbcAMP）或22R-羟基胆固醇存在下孵育时，也获得了类似的结果。在hCG或dbcAMP存在的情况下，与FI相比，FII中刺激系数（以类固醇产量计）更高。相反，FI中类固醇的基础产量高于FII，主要是睾酮。向莱迪希细胞孵育培养基中添加越来越多的生精小管培养基（STM），在基础和hCG刺激条件下，在两个富集莱迪希细胞组分中均导致类固醇产量呈剂量相关的增加。在生精小管长度增加的情况下也获得了类似的结果。用浓缩STM或生精小管与纯化的莱迪希细胞共培养进行的额外实验证实了生精分泌因子对莱迪希细胞类固醇生成存在旁分泌控制。来自生精小管的一种或多种旁分泌因子无论年龄如何，都对人类莱迪希细胞功能产生积极且高效的影响。
