Tsujinaka T, Ebisui C, Fujita J, Kishibuchi M, Morimoto T, Ogawa A, Katsume A, Ohsugi Y, Kominami E, Monden M
Department of Surgery II, Osaka University Medical School, Suita, Japan.
Biochem Biophys Res Commun. 1995 Feb 6;207(1):168-74. doi: 10.1006/bbrc.1995.1168.
Interleukin(IL)-6 transgenic mice were produced by microinjection of human IL-6 cDNA fused with H-2Ld promoter into the pronucleus of fertilized eggs from C57BL/6J mice. At 16 weeks old, the gastrocnemius muscles of the IL-6 transgenic mice became atrophic as compared to those of the normal mice, while the body weights increased significantly. The activities and mRNA levels of lysosomal cathepsins B and L were increased in the muscles of the transgenic mice. Immunohistochemical study on the muscles showed increased staining of both cathepsins B and L in the transgenic mice. IL-6 is responsible for enhanced muscle catabolism by activating the lysosomal cathepsin (B and L) system.
通过将与H-2Ld启动子融合的人白细胞介素6（IL-6）cDNA显微注射到C57BL/6J小鼠受精卵的原核中，制备了IL-6转基因小鼠。16周龄时，与正常小鼠相比，IL-6转基因小鼠的腓肠肌出现萎缩，而体重显著增加。转基因小鼠肌肉中溶酶体组织蛋白酶B和L的活性及mRNA水平升高。对肌肉的免疫组织化学研究显示，转基因小鼠中组织蛋白酶B和L的染色均增加。IL-6通过激活溶酶体组织蛋白酶（B和L）系统导致肌肉分解代谢增强。
