Mazzei M, Garzoglio R, Mariottini G L, Carli A
Istituto di Scienze Farmaceutiche, Università di Genova.
Boll Soc Ital Biol Sper. 1994 May-Jun;70(5-6):143-51.
HPLC separation of crude extract components derived from nematocysts and extranematocystic tissues of macroplanktonic jellyfish Aequorea aequorea and Rhizostoma pulmo and benthic sea-anemones Actinia equina and Anemonia sulcata was carried out by different columns. A satisfactory peak separation was obtained analyzing the toxin of Rhizostoma pulmo by cationic and C18 columns. Low molecular weight fragments were separated by C18 column and U.V. monitored varying pH values and obtaining the displacement of significant peaks. Clear differences between chromatographic plots concerning planktonic and benthic species was evidenced by anionic column; this result can point out a clear ecological analogy between species living in the same environment and a similar toxin biosynthesis, due to selective actions related to both the environment and the phylogenetic relationships; these organisms could have developed similar mechanisms useful to tackle the environment.
采用不同的色谱柱对大型浮游水母海月水母和肺形根口水母以及底栖海葵马氏海葵和沟迎风海葵的刺细胞和刺细胞外组织的粗提物成分进行了高效液相色谱分离。通过阳离子柱和C18柱分析肺形根口水母的毒素，获得了令人满意的峰分离效果。低分子量片段通过C18柱分离，紫外监测不同pH值并获得显著峰的位移。阴离子柱证明了浮游和底栖物种色谱图之间的明显差异；这一结果可以指出生活在同一环境中的物种之间存在明显的生态相似性以及类似的毒素生物合成，这是由于与环境和系统发育关系相关的选择性作用；这些生物可能已经发展出类似的机制来应对环境。
