Alderton M R, Korolik V, Coloe P J, Dewhirst F E, Paster B J
Department of Applied Biology and Biotechnology, Royal Melbourne Institute of Technology, Australia.
Int J Syst Bacteriol. 1995 Jan;45(1):61-6. doi: 10.1099/00207713-45-1-61.
Campylobacter hyoilei sp. nov. is the name proposed for an organism formerly described as strain RMIT 32AT (T = type strain) and a group of similar bacteria isolated from intestinal lesions of pigs with proliferative enteritis. The phenotypic characteristics of these organisms indicated that they are closely related to each other and are not strains of other Campylobacter spp. commonly isolated from pigs. The results of probing of ClaI-, EcoRV-, or BglII-cleaved genomic DNAs from C. hyoilei strains with a radiolabeled DNA probe that distinguishes between Campylobacter jejuni and Campylobacter coli indicated that C. hyoilei and C. coli are closely related. However, the 16S rRNA sequence of the reference strain of C. hyoilei, RMIT 32AT, was four bases different from the 16S rRNA sequence of C. jejuni CCUG 11284T and five bases different from the 16S rRNA sequence of C. jejuni subsp. doylei CCUG 24567T, suggesting that C. hyoilei is more closely related to C. jejuni than to C. coli. Hybridization between DNA from C. hyoilei type strain RMIT 32A and DNAs from selected type and reference strains of other Campylobacter species and subspecies, including C. jejuni, C. jejuni subsp. doylei, C. coli, Campylobacter mucosalis, and Campylobacter hyointestinalis, as well as the other C. hyoilei strains (the RMIT 32AT-like isolates), revealed that high levels of DNA hybridization (> 70%) occurred only between the reference strain and other strains of C. hyoilei.
猪源弯曲杆菌新种（Campylobacter hyoilei sp. nov.）是为一种先前被描述为RMIT 32AT菌株（T = 模式菌株）以及从患有增生性肠炎的猪的肠道病变中分离出的一组相似细菌所提议的名称。这些微生物的表型特征表明它们彼此密切相关，且不是通常从猪中分离出的其他弯曲杆菌属物种的菌株。用区分空肠弯曲杆菌和结肠弯曲杆菌的放射性标记DNA探针探测猪源弯曲杆菌菌株的ClaI、EcoRV或BglII切割的基因组DNA的结果表明，猪源弯曲杆菌和结肠弯曲杆菌密切相关。然而，猪源弯曲杆菌参考菌株RMIT 32AT的16S rRNA序列与空肠弯曲杆菌CCUG 11284T的16S rRNA序列有4个碱基不同，与空肠弯曲杆菌多伊氏亚种CCUG 24567T的16S rRNA序列有5个碱基不同，这表明猪源弯曲杆菌与空肠弯曲杆菌的关系比与结肠弯曲杆菌的关系更密切。猪源弯曲杆菌模式菌株RMIT 32A的DNA与其他弯曲杆菌物种和亚种（包括空肠弯曲杆菌、空肠弯曲杆菌多伊氏亚种、结肠弯曲杆菌、黏膜弯曲杆菌和猪肠弯曲杆菌）的选定模式菌株和参考菌株的DNA之间的杂交，以及其他猪源弯曲杆菌菌株（RMIT 32AT样分离株）之间的杂交，结果显示，只有参考菌株与其他猪源弯曲杆菌菌株之间出现了高水平的DNA杂交（> 70%）。
