Knapp W, Ludwig H
Wien Klin Wochenschr. 1976 Jan 9;88(1):1-5.
This report presents a review of recent developments in immunofluorescence and their resultant applications. After a short introduction to the basic principles of immunofluorescence, this study deals with newer developments in utilizable fluorochromes, new methods in the production of antisera and fluorochrome-labelled conjugates and recent improvements in the optical system. In particular, the necessity of suitable characterization and examination in regard to the quality of the conjugates is described in detail. A separate chapter has been devoted to the rapidly-developing quantitative immunofluorescence technique and its application.
本报告综述了免疫荧光技术的最新进展及其应用成果。在简要介绍免疫荧光基本原理之后,本研究探讨了可利用荧光染料的新进展、抗血清和荧光染料标记共轭物制备的新方法以及光学系统的最新改进。特别详细描述了关于共轭物质量进行适当表征和检测的必要性。单独成章介绍了快速发展的定量免疫荧光技术及其应用。