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[应用放射免疫定量分析法研究甲型流感病毒血凝素的抗原特异性]

[Antigenic specificity of influenza type A virus hemagglutinin by the method of radioimmunological quantitative analysis].

作者信息

Berezina O N, Blokha V V, Iamnikova S S, Iakhno M A, Zakstel'skaia L Ia

出版信息

Vopr Virusol. 1978 Mar-Apr(2):160-5.

PMID:78586
Abstract

The use of a test system o fa highly purified, antigenically homogeneus hemagglutinin of influenza A/MRC-11 virus and monospecific antiserum permitted to evaluate the potentials of competitive radioimmunoassay in model experiments investigating the antigenic characteristics and content of influenza A (H3N2) virus hemagglutinin. The method proved to be sufficiently specific to differentiate influenza virus strains by antigenic determinants of hemagglutinin, as well as highly sensitive, detecting very small (down to 1 ng) amounts of H3 hemagglutinin in a sample.

摘要

使用高度纯化、抗原性均一的甲型流感病毒A/MRC-11血凝素测试系统和单特异性抗血清,可在研究甲型(H3N2)流感病毒血凝素抗原特性和含量的模型实验中评估竞争性放射免疫测定的潜力。事实证明,该方法具有足够的特异性,可通过血凝素的抗原决定簇区分流感病毒株,并且灵敏度很高,能够检测样品中极少量(低至1纳克)的H3血凝素。

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