血浆中蒿甲醚及其代谢物双氢青蒿素的差异提取及高效液相色谱法测定
Differential extraction of artemether and its metabolite dihydroartemisinin from plasma and determination by high-performance liquid chromatography.
作者信息
Muhia D K, Mberu E K, Watkins W M
机构信息
Wellcome Trust Research Laboratories, Nairobi, Kenya.
出版信息
J Chromatogr B Biomed Appl. 1994 Oct 3;660(1):196-9. doi: 10.1016/0378-4347(94)00276-2.
A method is described for the separation of artemether (ARM) from its metabolite dihydroartemisinin (DHA) and determination by HPLC. The basis of the separation is differential extraction of the drugs from plasma as a function of plasma pH. Hexane extracted ARM from basiffied plasma and both ARM and DHA from normal plasma. Derivatized extracts were chromatographed on a 5-microns ODS column with water-acetonitrile (40:60) as mobile phase and detected at 254 nm. The method removes the need for expensive absorption cartridges (BondElut). Chromatography has been improved and the elution time shortened in comparison with previous methods.
描述了一种从蒿甲醚（ARM）及其代谢产物二氢青蒿素（DHA）中分离蒿甲醚并通过高效液相色谱法进行测定的方法。分离的基础是根据血浆pH值从血浆中对药物进行差异萃取。己烷从碱化血浆中萃取ARM，从正常血浆中萃取ARM和DHA。衍生化提取物在5微米的ODS柱上进行色谱分析，以水-乙腈（40:60）作为流动相，并在254纳米处进行检测。该方法无需使用昂贵的吸附柱（BondElut）。与以前的方法相比，色谱分析得到了改进，洗脱时间缩短。
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