Thomas C G, Ward S A, Edwards G
Department of Pharmacology and Therapeutics, University of Liverpool, UK.
J Chromatogr. 1992 Nov 27;583(1):131-6. doi: 10.1016/0378-4347(92)80355-t.
A sensitive and selective reversed-phase high-performance liquid chromatographic method for the determination of artemether and its major metabolite dihydroartemisinin in plasma has been developed. It involves extraction of plasma with dichloromethane, solid-phase separation of the two analytes and acid decomposition prior to chromatography on a C18 Spherisorb column with a mobile phase of acetonitrile-water (50:50, v/v). Run time is 30 min. The assay satisfies all of the criteria required for use in clinical pharmacokinetic studies.
已开发出一种灵敏且具选择性的反相高效液相色谱法，用于测定血浆中的蒿甲醚及其主要代谢物双氢青蒿素。该方法包括用二氯甲烷萃取血浆，对两种分析物进行固相分离以及在进行色谱分析前进行酸分解，色谱分析在C18 Spherisorb柱上进行，流动相为乙腈 - 水（50:50，v/v）。运行时间为30分钟。该测定法满足临床药代动力学研究所需的所有标准。
