Labroo R, Kharasch E D
Department of Anesthesiology, University of Washington, Seattle 98195.
J Chromatogr B Biomed Appl. 1994 Oct 3;660(1):85-94. doi: 10.1016/0378-4347(94)00263-0.
The short-acting synthetic opioid alfentanil undergoes extensive biotransformation to several metabolites. A gas chromatographic-mass spectrometric assay, using selected-ion monitoring and deuterated internal standards, was developed for quantitating the predominant metabolites of alfentanil. Optimal extraction and derivatization conditions are described. The assay was applied to the analysis of metabolites formed during alfentanil metabolism in vitro by human liver microsomes. Formation of known alfentanil metabolites was confirmed, and formation of a metabolite, not previously detected in vitro, is described. The assay represents a significant improvement over existing methods of alfentanil metabolite analysis, which use HPLC and radiochemical detection.
短效合成阿片类药物阿芬太尼会广泛生物转化为多种代谢产物。采用选择离子监测和氘代内标，开发了一种气相色谱-质谱分析法，用于定量阿芬太尼的主要代谢产物。文中描述了最佳提取和衍生化条件。该分析法应用于分析人体肝微粒体在体外阿芬太尼代谢过程中形成的代谢产物。已证实已知阿芬太尼代谢产物的形成，并描述了一种此前在体外未检测到的代谢产物的形成。与现有的使用高效液相色谱和放射化学检测的阿芬太尼代谢产物分析方法相比，该分析法有显著改进。
