Green P M, Giannelli F
Paediatric Research Unit, Prince Philip Research Labs, Guy's Hospital, London, UK.
Mol Biotechnol. 1994 Apr;1(2):117-24. doi: 10.1007/BF02921552.
This article describes the direct sequencing of PCR-amplified DNA, a technique that bypasses the problem of replication errors sometimes associated with other PCR procedures. The direct sequencing procedure produces an "average sequence" of all the copies of the target. Any miscopied molecule usually represents only a small proportion of the total. The technique described here is based on the "traditional" ddNTP sequencing method of Sanger et al.
本文描述了对聚合酶链反应（PCR）扩增的DNA进行直接测序的技术，该技术绕过了有时与其他PCR程序相关的复制错误问题。直接测序程序产生目标所有拷贝的“平均序列”。任何错误复制的分子通常仅占总数的一小部分。这里描述的技术基于桑格等人的“传统”双脱氧核苷酸（ddNTP）测序方法。
