藏红花素对苯并[a]芘引发的CD-1小鼠皮肤肿瘤促进作用的抑制
Inhibition of tumor promotion in benzo[a]pyrene-initiated CD-1 mouse skin by crocetin.
作者信息
Wang C J, Lee M J, Chang M C, Lin J K
机构信息
Institute of Biochemistry, Chung Shan Medical and Dental College, Taichung, Republic of China.
出版信息
Carcinogenesis. 1995 Feb;16(2):187-91. doi: 10.1093/carcin/16.2.187.
The effects of topical application of crocetin on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced promotion of skin tumors, hyperplasia, hydrogen peroxide, ornithine decarboxylase (ODC) and inflammation were evaluated in female CD-1 mice. Topical application of crocetin (0.2 or 1.0 mumol) with TPA (15 nmol) twice weekly for 20 weeks to mice previously initiated with benzo[a]pyrene (B[a]P) inhibited the number of TPA-induced tumors per mouse by 69 and 81% respectively. Pre-application of the same amount of crocetin also afforded significant protection against TPA-induced hyperplasia in the ear skin. Topical application of crocetin inhibited tumor promoter-caused induction of epidermal ODC activity by TPA (5 nmol). The topical application of crocetin (0.2 or 1.0 mumol) inhibited TPA-induced edema of mouse ears by 76 and 87% respectively. Pretreatment of mouse skin with various amounts of crocetin caused inhibition of hydrogen peroxide and myeloperoxidase production by TPA. These results indicate that crocetin possesses potential as a cancer chemopreventive agent against tumor promotion.
在雌性CD-1小鼠中评估了西红花酸局部应用对12-O-十四烷酰佛波醇-13-乙酸酯（TPA）诱导的皮肤肿瘤促进、增生、过氧化氢、鸟氨酸脱羧酶（ODC）和炎症的影响。将西红花酸（0.2或1.0 μmol）与TPA（15 nmol）每周两次局部应用于预先用苯并[a]芘（B[a]P）启动的小鼠，持续20周，可使每只小鼠TPA诱导的肿瘤数量分别减少69%和81%。预先应用相同量的西红花酸也能显著保护耳部皮肤免受TPA诱导的增生。局部应用西红花酸可抑制肿瘤启动剂TPA（5 nmol）引起的表皮ODC活性诱导。局部应用西红花酸（0.2或1.0 μmol）分别使TPA诱导的小鼠耳部水肿减少76%和87%。用不同量的西红花酸预处理小鼠皮肤可抑制TPA诱导的过氧化氢和髓过氧化物酶生成。这些结果表明，西红花酸具有作为肿瘤促进预防癌症化学预防剂的潜力。
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