Lee M J, Hsu J D, Wang C J
Institute of Biochemistry, Chung Shan Medical and Dental College, Taichung, Taiwan, R.O.C.
Anticancer Res. 1995 Mar-Apr;15(2):411-6.
The effects of topical application of geniposide on 12-o-tetradecanoylphorbol-13-acetate(TPA)-induced promotion of skin tumors, hyperplasia, ornithine decarboxylase (ODC) and inflammation were evaluated in female CD-1 mice. Topical application of geniposide (0.2 to 1.0 mumol) with TPA (15 nmol) twice weekly for 20 weeks to mice previously initiated with benzo[a]pyrene (B[a]P) inhibited the number of TPA-induced tumor per mouse by 84 or 89%, respectively. Pre-application of the same amount of geniposide also afforded significant protection against TPA-induced hyperplasia in the ear skin. Topical application of geniposide inhibited tumor promoter-caused induction of epidermal ODC activity by TPA (5 nmol). The topical application of geniposide (0.2 or 1.0 mumol) inhibited TPA-induced edema of mouse ears by 41 or 43%, respectively. Pretreatment of mouse skin with various amounts of geniposide caused inhibition of hydrogen peroxide (H2O2) and myeloperoxidase (MPO) formation by TPA. These results indicate that geniposide possesses potential as a cancer chemopreventive agent against tumor promotion.
在雌性CD-1小鼠中评估了京尼平苷局部应用对12-O-十四烷酰佛波醇-13-乙酸酯（TPA）诱导的皮肤肿瘤促进、增生、鸟氨酸脱羧酶（ODC）和炎症的影响。将京尼平苷（0.2至1.0 μmol）与TPA（15 nmol）每周两次局部应用于先前用苯并[a]芘（B[a]P）启动的小鼠，持续20周，分别使每只小鼠TPA诱导的肿瘤数量减少84%或89%。预先应用相同量的京尼平苷也能显著保护小鼠耳部皮肤免受TPA诱导的增生。局部应用京尼平苷可抑制肿瘤促进剂引起的TPA（5 nmol）诱导的表皮ODC活性。局部应用京尼平苷（0.2或1.0 μmol）分别使TPA诱导的小鼠耳部水肿减少41%或43%。用不同量的京尼平苷预处理小鼠皮肤可抑制TPA诱导的过氧化氢（H2O2）和髓过氧化物酶（MPO）的形成。这些结果表明，京尼平苷具有作为肿瘤促进预防癌症化学预防剂的潜力。
