Hellevuo K, Berry R, Sikela J M, Tabakoff B
Department of Pharmacology, University of Colorado Health Sciences Center, Denver 80262.
Hum Genet. 1995 Feb;95(2):197-200. doi: 10.1007/BF00209401.
A novel form of human adenylyl cyclase (ADCY7) has been discovered in the human erythroleukemia cell line (HEL). This cell line has been widely used as a model for studies of the characteristics of human platelets. Data from HEL cells suggests that ADCY7 may be the major AC form in human platelets. In the current study polymerase chain reaction (PCR) techniques coupled with use of human/rodent somatic hybrid panels and a yeast artificial chromosome (YAC) library were used to determine the chromosomal localization of the gene (adcy7) for ADCY7 enzyme. A 251-bp product from the 3' untranslated region of human adcy7 was amplified for PCR mapping and the results localize the adcy7 gene to region 16q12-16q13 of the human genome. The AC enzyme family is characterized by the presence of 12 membrane-spanning domains in its sequences, and this chromosomal region is known to contain other genes coding for proteins characterized by 12 membrane-spanning domains.
在人红白血病细胞系（HEL）中发现了一种新型的人腺苷酸环化酶（ADCY7）。该细胞系已被广泛用作研究人血小板特性的模型。来自HEL细胞的数据表明，ADCY7可能是人血小板中的主要腺苷酸环化酶形式。在当前研究中，聚合酶链反应（PCR）技术结合使用人/啮齿动物体细胞杂交板和酵母人工染色体（YAC）文库来确定ADCY7酶基因（adcy7）的染色体定位。从人adcy7的3'非翻译区扩增出一个251 bp的产物用于PCR定位，结果将adcy7基因定位于人类基因组的16q12 - 16q13区域。腺苷酸环化酶家族的特征是其序列中存在12个跨膜结构域，并且已知该染色体区域包含其他编码具有12个跨膜结构域蛋白质的基因。
