人类自身免疫抗原p80卷曲螺旋蛋白基因定位于染色体17q21 - q23，其可能的假基因定位于染色体14。

Assignment of a human autoimmune antigen, p80-coilin gene to chromosome 17q21-q23 and of its possible pseudogene to chromosome 14.

作者信息

Kusuda J, Tanuma R, Gotoh E, Toyoda A, Hashimoto K

机构信息

Division of Genetic Resources, National Institute of Health, Tokyo, Japan.

出版信息

Hum Genet. 1995 Feb;95(2):233-4. doi: 10.1007/BF00209411.

DOI:10.1007/BF00209411

PMID:7860074

Abstract

In order to determine the chromosomal locations of an autoimmune antigen, the coilin gene and its pseudogene, we amplified the segments of the two genes by the polymerase chain reaction (PCR) and screened a panel of somatic cell hybrids for the presence of the gene products. The results indicate that the human coilin gene and its pseudogene can be assigned to chromosome 17 and chromosome 14, respectively. Further analysis of cell hybrids bearing chromosome 17 with various deletions localized the coilin gene to the region q21-q23.

摘要

为了确定一种自身免疫抗原、卷曲螺旋蛋白基因及其假基因的染色体定位，我们通过聚合酶链反应（PCR）扩增了这两个基因的片段，并筛选了一组体细胞杂种以检测基因产物的存在。结果表明，人类卷曲螺旋蛋白基因及其假基因可分别定位于17号染色体和14号染色体。对携带不同缺失的17号染色体的细胞杂种进行进一步分析，将卷曲螺旋蛋白基因定位到q21-q23区域。