美国国立综合医学科学研究所人类/啮齿动物体细胞杂交定位板1和2。

NIGMS human/rodent somatic cell hybrid mapping panels 1 and 2.

作者信息

Drwinga H L, Toji L H, Kim C H, Greene A E, Mulivor R A

机构信息

NIGMS Human Genetic Mutant Cell Repository, Coriell Institute for Medical Research, Camden, New Jersey 08103.

出版信息

Genomics. 1993 May;16(2):311-4. doi: 10.1006/geno.1993.1190.

DOI:10.1006/geno.1993.1190

PMID:8314568

Abstract

The NIGMS Human Genetic Mutant Cell Repository is currently distributing two well-characterized human/rodent somatic cell hybrid mapping panels. Mapping Panel 1 consists of DNA isolated from 18 hybrid cell cultures retaining from 1 to 19 human chromosomes. Mapping Panel 2 contains DNA from hybrids retaining 1 or 2 human chromosomes. All but 2 of the hybrids retain a single intact human chromosome. Mapping Panel 2 is also available as cell cultures. These resources should prove valuable to the Human Genome Project initiative. This article describes the sources of the hybrid cell cultures and the procedures utilized to prepare and characterize the panels.

摘要

美国国立综合医学科学研究所人类遗传突变细胞库目前正在分发两个特征明确的人/啮齿动物体细胞杂交定位板。定位板1由从18种杂交细胞培养物中分离的DNA组成，这些培养物保留了1至19条人类染色体。定位板2包含来自保留1条或2条人类染色体的杂交体的DNA。除了2个杂交体外，所有杂交体都保留了一条完整的人类染色体。定位板2也可作为细胞培养物获得。这些资源对人类基因组计划应具有重要价值。本文描述了杂交细胞培养物的来源以及用于制备和鉴定定位板的程序。