通过聚合酶链反应（PCR）对NIGMS人/啮齿动物体细胞杂交定位板2进行表征。
Characterization of NIGMS human/rodent somatic cell hybrid mapping panel 2 by PCR.
作者信息
Dubois B L, Naylor S L
机构信息
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284-7762.
出版信息
Genomics. 1993 May;16(2):315-9. doi: 10.1006/geno.1993.1191.
PMID:8314569
Abstract
NIGMS human somatic cell hybrid mapping panel 2 was tested for specific markers for each human chromosome. Several discrepancies with the reported chromosome content were found by screening with PCR primers. Our results indicated that there were additional chromosomal fragments present in some of the cell lines and that a few of the hybrids had lost part of the reported chromosome.
摘要
对NIGMS人类体细胞杂交定位板2进行了各人类染色体特异性标记检测。通过用PCR引物筛选，发现了一些与报道的染色体组成存在差异的情况。我们的结果表明，一些细胞系中存在额外的染色体片段，并且一些杂交细胞失去了报道的部分染色体。
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