Gasparini G, Barbareschi M, Boracchi P, Bevilacqua P, Verderio P, Dalla Palma P, Ménard S
San Bortolo Medical Centre, Vicenza, Italy.
Int J Cancer. 1995 Mar 3;60(5):604-10. doi: 10.1002/ijc.2910600506.
Experimental studies have shown that the 67-kDa laminin receptor (LRec) is an important molecule for the interaction of tumour cells with the extracellular matrix, and that it plays a role in the early steps of angiogenesis and in tumour invasion and metastasis. We performed a multi-parametric study in 171 node-negative breast cancers, followed for a median time of 6 years, to verify whether determination of the LRec provides prognostic information in addition to intra-tumoral microvessel density (IMD), a measure of tumour angiogenesis, and to other biological and conventional indicators. A positive association between LRec expression and high neovascularization was found, although it did not reach significance. LRec was not associated either with other biological markers (oestrogen receptor, progesterone receptor and p53 expression) or to the conventional prognostic features [menopausal status, tumour size, histological types, grading and peri-tumoral lymphatic vessel invasion (PLVI)]. In univariate analysis, IMD, p53, PgR, PLVI, grading and tumour size were significant prognostic indicators of relapse-free survival (RFS). LRec expression approached significance when considered as a dichotomous variable, after having selected the optimum cutoff of 10% to distinguish high-risk from low-risk patients. For overall survival (OS), tumour size and IMD (continuous variable) were significant prognostic factors, and p53 approached significance. In multivariate analysis for RFS, the joint variable LRec and vascularization was the strongest independent prognostic factor, followed by PgR, PLVI and p53. For OS, tumour size was the only independent prognostic indicator in this series.
实验研究表明，67 kDa层粘连蛋白受体（LRec）是肿瘤细胞与细胞外基质相互作用的重要分子，并且在血管生成的早期步骤以及肿瘤侵袭和转移中发挥作用。我们对171例腋窝淋巴结阴性乳腺癌患者进行了一项多参数研究，随访时间中位数为6年，以验证LRec的检测除了肿瘤内微血管密度（IMD，一种肿瘤血管生成的指标）以及其他生物学和传统指标外，是否能提供预后信息。尽管未达到显著水平，但发现LRec表达与高新生血管形成之间存在正相关。LRec与其他生物学标志物（雌激素受体、孕激素受体和p53表达）或传统预后特征[绝经状态、肿瘤大小、组织学类型、分级和肿瘤周围淋巴管浸润（PLVI）]均无关联。在单因素分析中，IMD、p53、PgR、PLVI、分级和肿瘤大小是无复发生存期（RFS）的显著预后指标。在选择10%的最佳临界值以区分高危和低危患者后，将LRec表达作为二分变量考虑时接近显著水平。对于总生存期（OS），肿瘤大小和IMD（连续变量）是显著的预后因素，p53接近显著水平。在RFS的多因素分析中，联合变量LRec和血管生成是最强的独立预后因素，其次是PgR、PLVI和p53。对于OS，肿瘤大小是本系列中唯一的独立预后指标。
