Suescun M O, Calandra R S, Lustig L
Instituto Multidisciplinario de Biología Celular, La Plata, Argentina.
J Androl. 1994 Sep-Oct;15(5):442-8.
The endocrinological profile of animals with experimental autoimmune orchitis (EAO) has not been sufficiently explored. With this purpose orchitis was induced in adult rats by active immunization with testicular homogenate (TH) and adjuvants. Animals were sacrificed 50 or 80 days after the first immunization. Forty-three percent of rats immunized with TH developed orchitis. Different degrees of cell sloughing and atrophy of the seminiferous tubules and numerous macrophages and lymphocytes in close association with Leydig cells were seen. A significant increase in the number of Leydig cells was observed in rats with orchitis killed at 50 and 80 days. An enhanced number of interstitial non-Leydig cells was also detected in rats with testicular damage killed at 80 days. Levels of serum follicle-stimulating hormone (FSH) were two- to threefold higher in rats with EAO compared to concentrations detected in other groups. Moreover, rats with orchitis had significantly increased testicular testosterone. Serum luteinizing hormone (LH) did not change in animals of any group. In vitro studies showed an increase in the basal and human chorionic gonadotropin (hCG)-stimulated testosterone production in rats with EAO. The increase in testicular steroidogenesis without a concomitant enhancement in serum LH levels detected in rats with autoimmune orchitis suggests the existence of local control mechanisms.
实验性自身免疫性睾丸炎（EAO）动物的内分泌特征尚未得到充分研究。为此，通过用睾丸匀浆（TH）和佐剂进行主动免疫，在成年大鼠中诱发睾丸炎。首次免疫后50天或80天处死动物。用TH免疫的大鼠中有43%发生了睾丸炎。可见不同程度的生精小管细胞脱落和萎缩，以及与睾丸间质细胞紧密相关的大量巨噬细胞和淋巴细胞。在50天和80天处死的患睾丸炎大鼠中，观察到睾丸间质细胞数量显著增加。在80天处死的睾丸受损大鼠中，还检测到间质非睾丸间质细胞数量增加。与其他组相比，EAO大鼠的血清促卵泡激素（FSH）水平高出两到三倍。此外，患睾丸炎的大鼠睾丸睾酮显著增加。任何组的动物血清促黄体生成素（LH）均未发生变化。体外研究表明，EAO大鼠基础和人绒毛膜促性腺激素（hCG）刺激的睾酮生成增加。在自身免疫性睾丸炎大鼠中检测到睾丸类固醇生成增加而血清LH水平未随之升高，这表明存在局部控制机制。
