枯草芽孢杆菌硝酸盐同化和厌氧生长所需基因的鉴定与分离。
Identification and isolation of a gene required for nitrate assimilation and anaerobic growth of Bacillus subtilis.
作者信息
Glaser P, Danchin A, Kunst F, Zuber P, Nakano M M
机构信息
Unité de Régulation de l'Expression Génétique, Institut Pasteur, Paris, France.
出版信息
J Bacteriol. 1995 Feb;177(4):1112-5. doi: 10.1128/jb.177.4.1112-1115.1995.
Abstract
The Bacillus subtilis narA locus was shown to include narQ and narA. The putative product of narQ is similar to FdhD, which is required for formate dehydrogenase activity in Escherichia coli. NarA showed homology to MoaA, a protein involved in biosynthesis of the molybdenum cofactor for nitrate reductase and formate dehydrogenase. Analysis of mutants showed that narA but not narQ is required for both nitrate assimilation and respiration.
摘要
枯草芽孢杆菌narA基因座被证明包含narQ和narA。narQ的推定产物与FdhD相似，FdhD是大肠杆菌中甲酸盐脱氢酶活性所必需的。NarA与MoaA具有同源性，MoaA是一种参与硝酸还原酶和甲酸盐脱氢酶钼辅因子生物合成的蛋白质。对突变体的分析表明，硝酸盐同化和呼吸作用都需要narA而不是narQ。
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